摘要
目的:探讨原发性食管鳞癌(esophageal squamous cell carcinoma,ESCC)组织中mir-183的表达状况及其与食管鳞癌临床病理特征之间的关系.方法:应用实时RT-PCR方法及2-△△CT分析法分别检测ESCC患者癌组织及癌旁正常组织中mir-183的表达状况及其与临床病理特征的相关性.结果:在53例标本中,有22例(41.51%)mir-183高表达(2-6411倍),mir-183的高表达分别与患者的淋巴结转移情况及不良预后有显著统计学意义(P<0.05).结论:mir-183的高表达在ESCC的发生发展中发挥重要作用.
AIM：To detect the expression of miR-183 in primary esophageal squamous cell carcinoma（ESCC） and to analyze its correlation with clinicopathological features of ESCC.METHODS：The expression of miR-183 in 53 ESCC samples and matched tumor-adjacent tissue samples was detected by real-time PCR.PCR data were analyzed using the 2-ΔΔCT method.RESULTS：Of the 53 ESCC samples analyzed,microRNA-183 was significantly up-regulated（2-6411 folds） in 22 samples compared to the matched tissue samples.Up-regulation of mir-183 expression was correlated with lymph node metastasis and poor prognosis in ESCC（both P0.05） .CONCLUSION：Up-regulation of mir-183 expression was significantly correlated with the development and progression of ESCC.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第31期3246-3249,共4页
World Chinese Journal of Digestology
基金
浙江省自然基金资助项目
No.Y2080749
Y2091110
浙江省人事部优秀留学回国人员科技活动项目择优基金资助项目
No.2008A004
浙江省科技计划基金资助项目
No.2009C33143
教育部优秀留学回国人员科研基金资助项目
No.2010609
卫生部科研基金资助项目
No.WKJ2010-2-004
浙江省重大科技专项计划基金资助项目
No.2011C13039-1~~
关键词
Mir-183
食管鳞癌
临床病理特征
预后
MiR-183
Esophageal squamous cell carcinoma
Clinicopathological features
Prognosis
