锰超氧化物岐化酶在大肠癌中的表达及临床病理相关性分析

Expression of Manganese Superoxide Dismutas in Colorectal Carcinoma and Its Relationship with Clinicopathological Findings

目的探讨锰超氧化物岐化酶(MnSOD)在大肠癌组织中的表达及其临床病理学意义。方法采用免疫组织化学方法检测25例大肠癌、大肠腺瘤、癌旁组织中MnSOD表达及细胞定位,比较大肠癌、癌旁组织、大肠腺瘤中MnSOD的阳性率;RT-PCR方法检测20例大肠癌及癌旁组织,10例大肠腺瘤中MnSOD的水平,比较大肠癌、癌旁组织和大肠腺瘤中MnSOD mRNA相对表达量,分析大肠癌组织中MnSOD表达水平与各临床参数之间的关系。结果大肠癌癌组织、大肠腺瘤组织及癌旁正常大肠组织中MnSOD表达率分别为76%、44%及16%,大肠癌中MnSOD表达率显著高于癌旁正常大肠组织和大肠腺瘤组织(P均<0.05)。MnSOD在大肠癌中的表达和组织分化程度有关(P<0.05),与其他临床病理因素无关(P>0.05)。结论 MnSOD可能在大肠癌发生、发展中发挥作用,有望成为大肠癌潜在的生物标志物。

Objective To explore the expression of manganese superoxide dismutas（MnSOD） in colorectal carcinoma and its relationship with the clinicopathological findings.Methods The expressions of MnSOD in colorectal carcinoma,adenoma,and adjacent corresponding intestinal mucosal tissues were detected by immunohistochemistry and reverse transcription-polymerase chain reaction（RT-PCR）.The relationship between MnSOD expression level in colorectal adenoma and clinical parameters was analyzed.Results The expression of MnSOD was negative in adjacent corresponding colorectal tissues.The positive expression rate of MnSOD was 44%（11/25）in colorectal adenoma and 76%（19/25）in colorectal carcinoma（P0.05 when compared with the colorectal adenoma and its adjacent tissues）.The expression of MnSOD was positively correlated with histopathobigical grades（P0.05） but not with other clinicopathological findings（P0.05）.Conlusion The expression of MnSOD may be associated with the carcinogenesis and progression of colorectal carcinoma,and therefore may be used as a new biomarker.