川芎嗪对肝创伤后早期大鼠残肝组织保护作用的评价

Protective effect of ligustrazine on residual liver tissue in rats after hepatectomy

目的:研究川芎嗪干预对68%(2/3)肝切除大鼠术后残肝组织的保护作用,以及其对肝脏核因子κB(NF-κB)激活的影响.方法:60只SD大鼠随机分成3组(每组20只),均行68%(2/3)肝切除术.术后30 min分别给予以下处理:A组腹腔注射生理盐水,B组注射吡咯烷二硫代氨甲基甲酸盐(PDTC),C组注射川芎嗪.术后2 h、6 h、10 h和24 h观察各组大鼠术后一般情况和血清转氨酶变化.组织切片观察残肝组织病理状况,Western-blot方法检测NF-κB的激活.结果:C组大鼠残肝组织切片细胞水肿较A组轻:丙氨酸氨基转氨酶(ALT)在6 h(488.9 U/L±59.2 U/L)、10 h(670.0 U/L±73.4 U/L),较A组(651.6 U/L±65_3 U/L,930.0 U/L±62.9 U/L)低,P<0.05;天门冬氨酸氨基转移酶(AST)在6 h(11113.1 U/L±138.7 U/L)、10 h(1388.2 U/L±209.6 U/L),较A组(1315.0 U/L±111.0 U/L,1728.4 U/L±87_3 U/L)低,P<0.05:NF-κB的激活在术后2 h(0.78±0.04)和10 h(0.75±0.07)均低于A组(均为1),高于B组(0.68±0.09,0.66±0.04),P<0.05,6 h(0.71±0.07)低于A组(1),P<0.05,与B组(0.64±0.09)接近,P>0.05.结论:川芎嗪通过抑制NF-κB的激活,对创伤后肝组织产生保护作用,其保护作用接近于PDTC.

AIM：To investigate the effect of ligustrazine in alleviating inflammation and inhibiting the activation of NF-κB in rats after liver trauma. METHODS：Sixty rats which underwent 2/3 hepatectomy were randomly and equally divided into three groups.Group A was intraperitoneally injected with normal saline,and groups B and C were injected with PDTC and ligustrazine, respectively.The general status of the rats was observed,and changes in serum levels of aminotransferases were measured.Hepatic pathological changes were examined,and the activation of NF-κB was investigated by Western blot. RESULTS：Cellular swelling was milder in group C than in group A.Serum levels of ALT at 6 and 10 h after the operation were significantly lower in group C than in group A（6 h：488.9 U/L±59.2 U/L vs 651.6±65.3 U/L;10 h：670.0 U/L±73.4 U/L vs 930.0 U/L±62.9 U/L;both P 0.05）. Serum levels of AST at 6 and 10 h were also significantly lower in group C than in group A（6 h： 1113.1 U/L±138.7 U/L vs 1315.0 U/L±111.0 U/L; 10 h：1388.2 U/L ＋ 209.6 U/L vs 1728.4 U/L±87.3 U/L;both P 0.05）.The levels of activated NF-κB in group C（0.78±0.04,0.75±0.07） were lower than those in group A（both were 1）,higher than those in group B（0.68±0.09,0.66±0.04） at 2 and 10 h（all P 0.05）,but were comparable to that in group B at 6 h（0.71±0.07 vs 0.64±0.09,P 0.05）. CONCLUSION：Ligustrazine protects the post-traumatic liver tissue possibly by inhibiting the activation of NF-κB.