硅对稻瘟病菌侵染下水稻叶片超微结构的影响

Effects of Silicon on the Ultrastructure of Oryza sativa Leaves Infected by Magnaporthe grisea

为进一步揭示硅对稻瘟病的结构抗性,以纳米SiO2为硅源,以对水稻稻瘟病Magnaporthe grisea具有抗病性的农大18号和感病性的蒙古稻为材料,借助扫描电镜和透射电镜,在接种稻瘟病菌后对2个品种水稻叶片进行电镜观察.试验结果表明,接种稻瘟病菌后,无论是抗病品种还是感病品种,经过施硅处理的水稻叶片表面只存在少数的稻瘟病菌菌丝,并且其菌丝数量要比不施硅处理少.通过透射电镜图片还可看到,在水稻叶片内形成的矿化硅材料将病菌菌丝包围,减少了稻瘟病菌对水稻的侵染.施硅能够有效地抑制稻瘟病菌的入侵,从而增强水稻对稻瘟病的结构抗性.

To investigate the resistant effect of silicon on leaves of two rice varieties with nanometer SiO2 inoculation treatment against Magnaporthe grisea, resistant variety Nongda 18 to M. grisea and susceptible variety Mongolian rice to the pathogenic fungus were observed with SEM and TEM compared with control groups without the SiO2 treatment. The findings showed there were only a few hyphae of M. grisea on both leaves, and less hypha quantities than control group without Si treatment. Based on TEM pictures, it was clearly observed that Si might prevent the hypha from penetration by enclosing hypha around sites penetrated. Consequently, the result indicated that nanometer SiO2 was able to protect rice from the pathogentic fungus.