摘要
以烟73酿酒葡萄为花色苷提取材料,采用HPLC分析方法,对10种树脂的吸附/解析效果进行了比较。选用性能较优的XAD-7HP树脂进行纯化实验并对纯化后花色苷的稳定性进行了研究。结果表明,纯化处理使花色苷在干物质中的含量由处理前的48.16%提高到处理后的94.93%,色价由处理前的47.7提高到处理后的291.6,回收率为85.7%。花色苷的稳定性实验结果表明,随着光照强度、温度、pH值的升高,花色苷的不稳定性明显加剧。在避光、pH值为3,4℃下存放7 d,花色苷的保留率在95%以上,适宜于花色苷的短期保存。
Anthocyanins was extracted from Yah73 grape and then purified by ten kinds of resins and the absorption effects were compared by use of HPLC. The selected XAD-7HP resin with better purifying performance was used for anthocyanins purification and the stability of the purified anthocyanins was studied. The results indicated that anthocyanins content in dry substances increased from 48.16 % to 94.93 % after purification treatment and its color scale increased from 47.7 to 291.6 with the recovery as 85.7 %. The stability experimental results of anthocyanins suggested that anthocyanins became more unstable evidently with the increase of light intensity, temperature, and pH values. As stored at pH 3, 4 ℃ and in dark in one week, the retension rate of anthocyanins was above 95 %. Accordingly, such conditions were suitable for short storage of anthocyanins.
出处
《酿酒科技》
北大核心
2012年第1期28-31,共4页
Liquor-Making Science & Technology
基金
泰山学者"建设工程
