嗜麦芽寡养单胞菌DHHJ固态发酵羽毛工艺的研究

Study on solid-state fermentation of feather with Stenotrophomonas maltophilia DHHJ

为了将微生物处理羽毛工艺产业化,利用课题组筛选得到的嗜麦芽寡养单胞菌,在液态发酵羽毛工艺的基础上进行固态发酵工艺的研究。通过发酵条件和培养基成分的优化,确定了嗜麦芽寡养单胞菌固态发酵羽毛的工艺为:初始pH为7.5,培养温度为35℃,培养时间为4 d,培养基组成是麸皮和干酪素,固液比是1∶1.5,照此工艺发酵,发酵液中蛋白质含量为23.78 mg/mL,角蛋白酶活为20.46 U/mL,菌株的产酶能力及降解羽毛能力显著高于液态发酵条件。

In order to industrialize the utilization of waste feather by microbiological method,the test of solid-state fermentation for feather with Stenotrophomonas maltophilia DHHJ was studied based on its liquid-state fermentation previously.The results showed that the optimal solid state fermentation was initial pH 7.5 at 35 ℃ for four days,the medium was composed of bran and casein,and the solid-liquid ratio was 1∶ 1.5.Under the optimal conditions,the abilities of keratinase production and feather degradation of strain DHHJ were much better than those under the liquid-state fermentation,the protein content was 23.78 mg/mL and the activity of keratinase was 20.46 U/mL.