苦味叶下珠的化学成分研究

Chemical constituents of Phyllanthus amaus

目的对苦味叶下珠Phyllanthus amarus的化学成分进行研究。方法采用硅胶柱色谱、Sephadex-20柱色谱、重结晶及制备高效液相色谱等技术提取分离苦味叶下珠的化学成分,采用UV、IR、EI-MS、1H-NMR、13C-NMR、HMQC及HMBC等光谱方法鉴定化合物结构。结果从苦味叶下珠乙醇提取物的石油醚部位分离得到化合物1～3,分别鉴定为棕榈酸(1)、对羟基苯甲醛(2)、齐墩果酸(3);从二氯甲烷部位分离得到化合物4～9,分别鉴定为stigmast-5-en-3-ol,oleate(4)、4-羟基-3-甲氧基苯甲酸(5)、胡萝卜苷(6)、芹菜素(7)、木犀草素(8)、叶下珠新苷(9)。结论化合物9是新化合物,命名为叶下珠新苷,化合物4～8为首次从苦味叶下珠中分离得到。

Objective To study the chemical constituents of Phyllanthus amarus.Methods Chromatography on silica gel column,Sephadex LH-20 columm,recrystallization,and preparative HPLC technique were used to isolate and purify the compounds.Spectroscopic methods including UV,IR,EI-MS,1^H-NMR,13^C-NMR,HMQC,and HMBC were used to elucidate the structures of compounds.Results Nine compounds were obtained and identified as palmitic acid（1）,4-hydrobenzal dehyde（2）,oleanolic acid（3）,stigmast-5-en-3-ol,oleate（4）,4-hydroxy-3-methoxy-benzoic acid（5）,daucosterol（6）,apigenin（7）,luteolin（8）,and phyllanoside（9）.Conclusion Compound 9 is a new compound,named phyllanoside.Compounds 4—8 are isolated from the plant for the first time.