叶下珠种质资源遗传多样性的ISSR分析

ISSR analysis on genetic diversity in Phyllanthus urinaria

目的对分布于我国30个居群的叶下珠进行遗传多样性分析。方法采用ISSR分子标记技术,研究来自我国重庆、陕西、河南、云南、广西、广东、浙江、贵州、福建、海南省区共30个叶下珠居群的遗传多样性。结果从60个ISSR引物中筛选出24个用于扩增,共扩增得到264条条带,其中共有条带19条,多样性条带245条,多态位点百分率(PPB)为92.80%,遗传相似性系数(GS)值在0.579 5～0.916 7,多态性较高。UPGMA聚类结果显示我国叶下珠居群大致可以分为3支,即内陆分支、沿海分支及位于云南省区的过渡分支;个别居群呈现明显的居群特异性。结论叶下珠种质资源遗传多样性较高。

Objective To detect the genetic diversity of 30 populations of Phyllanthus urinaria from some provinces of China.Methods ISSR was used to analyze the genetic diversity of 30 populations of P.urinaria from Chongqing,Shaanxi,Henan,Yunnan,Guangxi,Guangdong,Zhejiang,Guizhou,Fujian,and Hainan Provinces of China.Results Twenty four primers selected from 60 ISSR primers were used for amplification and a total of 264 DNA bands were obtained,including 245 polymorphic bands and 19 common bands.At species level,the average percentage of polymorphic bands（PPB） was 92.80%.The range of the genetic similarity（GS） value was 0.579 5-0.916 7,with a higher genetic diversity.Cluster analysis showed that the P.urinaria population was roughly divided into three branches： inland branch,coastal branch,and transition branch which located in Yunnan Province;Individual populations presented obvious specificity.Conclusion The genetic diversity of germplasm resources in P.urinaria is higher.