金钻蔓绿绒组培再生体系的建立

Establishment of Tissue Culture Regeneration System for Philodendron con-go

以金钻蔓绿绒幼苗茎段为外植体,进行愈伤组织诱导、不定芽分化及增殖培养的研究。结果表明:金钻蔓绿绒茎段在MS+6-BA 5.0mg/L+NAA 0.1mg/L培养基可成功诱导出愈伤组织,继而分化出不定芽;不定芽适宜增殖培养基为MS+6-BA 6.0mg/L+NAA 0.2mg/L,增殖倍数可达3.93;不定芽适宜的生根培养基为1/2MS+NAA 1.0mg/L,生根率可达100%,移栽30d后成活率达95%以上。

Tissue culture regeneration system of Philodendron con-go was established successfully on using stem segments which from young plants as explant to study callus tissue induction, adventitive bud differentiation, proliferation culture explants. The results showed that the most suitable medium of inducation callus tissue and differentiation of adventitions bud was MS＋6-BA 5. 0 mg/L＋NAA 0. 1 mg/L; suitable medium of multiplication culture was MS＋6-BA 6. 0 rag/L＋ NAA 0. 2 rag/L, and the multiplication rate reached to 3. 93; rooting medium was 1/2 MS＋NAA 1.0 rag/L, rooting rate of adventitious bud was 100% ,and survival rate was more than 95% after 30 days transplanting.