采用RAPD技术筛选马口鱼性别差异相关分子标记

Apply RAPD to screen out sex differentiation related molecular marker in Opsariichthys bidens

选用SBSA和SBSB两组随机引物(各20条)分别对马口鱼卵巢DNA和精巢DNA进行RAPD扩增,并用1.5%的琼脂糖凝胶电泳检测扩增结果。结果表明,在40条随机引物中,有11条引物可以扩增出明显的雌雄性别差异条带,条带范围以SBSA5、SBSA6、SBSA9、SBSA16、SBSA19、SBSA20、SBSB2、SBSB3、SBSB9、SBSB16、SBSB17为主。运用RAPD筛选获得马口鱼性别DNA标记将有助于了解其调控雌雄性别发生的差异基因,为进一步的遗传育种提供基础。

Two groups （20 for each） of random primers （SBS A and SBS B） were used to RAPD apply in spawner＇s ovary and milter＇s spermary of Opsariichthys bidenas in this paper, and the products of PCR amplification were detected by 1.5% agarose gels electrophoresis. The results showed that 11 in 40 primers of PCR could apply obvious gender differences strips between male and female, others were primarily SBS A5, SBS A6, SBS A9, SBS A16, SBS A19, SBS A20, SBS B2, SBS B3, SBS B9, SBS B16, SBS B17. The results indicated that the screening for fish DNA markers will help to understand gender occur and their gene regulation in Opsariichthys bidenas, and further provide the basis for genetic breeding.