太湖光合自养真核超微藻遗传多样性初探

Genetic diversity of photosynthetic picoeukaryotes in Lake Taihu

采用真核藻类叶绿体16S rRNA的引物PLA491F、OXY1313R,通过PCR、构建基因克隆库的方法初步调查了太湖梅梁湾和东太湖光合自养真核超微藻的遗传多样性.结果表明,太湖自养型真核超微藻主要为隐藻(Cryptophyta),其次为硅藻(Bacillariophyta)、金藻(Chrysophyta)和定鞭藻(Haptophyta).该结果有助于我们更全面地了解太湖浮游藻类的群落结构及生态功能.

Genetic diversity of photosynthetic picoeukaryotes has been investigated at two stations located in Meiliang Bay and East Taihu in Lake Taihu, respectively. Genetic librat4es of chloroplast 16S rRNA gene have been constructed by using primer set PLA491F/OXY1313R, biased towards chloroplast of marine algae. The results show that most of photosynthetic picoeukaryotic se- quences belong to Cryptophyta, and the rest of them are affiliated with Bacillariophyta, Chrysophyta and Haptophyta. The study can help us take knowledge of the community structure and ecological function of phytoplankton in Lake Taihu.