寻常性银屑病患者血清高迁移率蛋白和血管内皮细胞钙黏蛋白的含量变化

Clinical significance of serum high mobility group box-1 and VE-cadherin in patients with psoriasis vulgaris

目的 探讨血清高迁移率蛋白1（ HMGB1）和血管内皮细胞钙黏蛋白（VE-cadherin）含量变化在寻常性银屑病发病中的作用及其临床意义.方法 应用双抗体夹心酶联免疫吸附法（ ELISA）检测78例寻常性银屑病患者外周血清中HMGB1和VE-cadherin的水平；并动态观察治疗前后HMGB1和VE-cadherin的变化及其与寻常性银屑病患者病情活动指标之间的关系.结果 寻常性银屑病患者血清中HMGB1和VE-cadherin的水平[分别为（2.58 ±0.19）、（4.22±0.81） mg/L]较正常对照组[分别为（0.86±0.12）、（2 87±0.64）mg/L]明显增高（t=10.53,10.16,P＜0.01）.进行期与静止期的寻常性银屑病患者血清中HMGB1和VE-cadherin水平[进行期患者分别为（3.58±0.17）、（5.62±0.85） mg/L,静止期分别为（2.07±0.12）、（3.45±0.82） mg/L]差异有统计学意义（t=12.47,13.11,P＜0.01）.治疗中、治疗后血清HMGB1、VE-cadherin水平及银屑病面积与严重性指数（PASI）评分与治疗前相比明显下降[治疗前分别为（2.58±0.19）、（4.22±0.81） mg/L,（12.0±4.2）分；治疗中分别为（1.98±0.17）、（3.45±0.79） mg/L,（8.38±3.49）分；治疗后分别为（0.92±0.19）、（2.94±0.65） mg/L,（2.67±0.87）分],差异有统计学意义（治疗中与治疗前相比,t=7.48、7.92、6.67,P＜0.05；治疗后与治疗前相比,t=12.72,11.48,13.13,P＜0.01）.治疗后与对照组相比,HMGB1和VE-cadherin的水平差异无统计学意义.寻常性银屑病患者治疗前后血清HMGB1和VE-cadherin水平及银屑病PASI评分呈明显正相关（r=0.58,0.64,P＜0.01）；同时VE-cadherin水平与银屑病PASI评分呈明显正相关（r=0.53,P＜0.01）.结论 HMGB1和VE-cadherin在寻常性银屑病的发病中可能起重要作用,血清HMGB1和VE-cadherin的检测可作为反映寻常性银屑病病情活动的指标之一.

Objective To investigate the role and clinical significance of serum levels of high mobility group box-1 （ HMGB1 ） and VE-cadherin in patients with psoriasis vulgaris.Methods Serum levels of HMGB1 and VE-cadherin were measured by double antibody sandwich enzyme-linked immunosorbent assay （ELISA） in 78 patients with psoriasis vulgaris.Results The serum levels of HMGB1 and VE-cadherin before treatment were significantly higher than those after treatment and those in normal controls （P 〈0.01 ）.There were significant differences between the active stage and the resting stage of psoriasis vulgaris （t =12.47,13.11,P 〈 0.01 ）.The serum levels of HMGB1 were associated with VE-cadherin and PASI scores（r=0.58,0.64,P〈0.01）.Conclusion The HMGB1 and VE-cadherin may affect the pathogenesis of psoriasis vulgaris.