SD大鼠重症肌无力被动转移模型的建立

Model of passive transferred experimental myasthenia gravis inducing on Sprague-Dawley rats

目的探讨采用SD大鼠建立重症肌无力(MG)被动转移模型的可能性。方法采用杂交瘤细胞株制备乙酰胆碱受体单克隆抗体mAb35,并测定其蛋白含量和纯度,用TE671细胞株行免疫细胞化学进行免疫学鉴定。用提纯后mAb35腹腔注射SD大鼠后观察其临床症状,用α-银环蛇毒检测大鼠神经-肌肉接头处乙酰胆碱受体数量变化,并用透射电镜观察神经-肌肉接头处超微结构变化,并与生理盐水注射对照组相比较。结果 SD大鼠经mAb35注射后均出现典型MG的肌无力表现,神经-肌肉接头处出现乙酰胆碱受体数量减少,神经-肌肉接头处突触皱褶变少,突触间隙变大等与临床MG类似的病理变化。结论 SD大鼠可成功用于建立MG被动转移模型,可广泛用于MG实验研究。

Objective To investigate the possibility of building the passive transferred experimental myasthenia gravis model in Sprague-Dawley（SD） rats.Methods Hybridoma cell lines were adopted to produce acetylcholine receptor（AChR） monoclonal antibody-mAb35,and the purity and content of the produced monoclonal antibody-mAb35 were detected.Immunocytochemistry method and TE671 cells were conducted to detect the immunological characters of the antibody.Symptoms were observed after intraperitoneally injecting purified monoclonal antibody-mAb35 to SD rats.The amount of AChRs marked by alpha bungarotoxin was detected.Ultrastructural changes of neuromuscular junction were observed by transmission electron microscope,and were compared with the control group（intraperitoneally injected with normal saline）.Results All the SD rats injected with monoclonal antibody-mAb35 showed symptoms similar to myasthenia gravis.The amount of AChR,which was marked by bungarotoxin,decreased in their neuromuscular junctions,and the ultrastructure of neuromuscular junctions in these models showed particular pathological changes similar to clinical myasthenia gravis,such as the less synaptic folds and the larger synaptic gaps.Conclusions Our findings suggested that the SD rats were a successful selection on building the passive transferred experimental myasthenia gravis model.It could be widely used in myasthenia gravis research.