生物传感器在脂肪酶酯化反应过程中的应用

Application of biosensor in the process of lipase esterification

为更准确地反映脂肪酶在催化酯化反应过程中的变化规律,设计、研制了将脂肪酶催化底物所产生的电子转移过程转换为电流输出的电化学生物传感器。分别以Novozym 435和Lipozyme TLIM作为生物元件,采集65℃下共轭亚油酸与甘油酯化反应过程的电流变化,用液相色谱仪测定反应过程中底物的含量,验证电流变化过程与酯化反应过程的吻合程度。通过实验证明相对误差为4.7%。证明了通过检测反应过程的电流变化可以判断酶催化底物反应的程度,为实现计算机连续在线检测控制反应过程提供理论基础。

An electrochemical biosensor was designed and developed which could transform the electron metastasis process produced by lipase conjugating the substrate to current output in order to accurately reflect the diversification of regular pattern of lipase in the process of catalytic esterification.Novozym 435 and Lipozyme TL IM were used as biological components respectively, electric current in the process of esterification of CLA and glycerin was collected, liquid chromatography was used to determine the content of the substrate of the reaction which could verify the degree of agreement between the process of current changing and esterification.The relative error was 4.7% by making experiments.It proved that we could judge the extent of the esterification conjugated by lipase by detecting the current changing in the process of the reaction which provided a basic premise for realizing the continuous detection on-line by computer.