茶树菇多糖的提取、气相色谱分析和AFM观测以及活性研究

Study on the extraction,GC analysis,AFM observation and activity of polysaccharide from Agrocybe aegerita

利用水提得到茶树菇水提多糖S-ACP,并对水提残渣进行碱提得多糖J-ACP。分别用考马斯亮蓝G-250法及苯酚硫酸法测定蛋白含量及总糖含量,气相色谱(GC)分析多糖组分,原子力显微镜(AFM)观测结构形貌,并进行了体外抗氧化活性测定。结果表明,S-ACP的单糖组成为木糖、甘露糖、葡萄糖、半乳糖,其摩尔比为2.527:0.140:1.284:1.738;J-ACP的单糖组成为鼠李糖、木糖、甘露糖、葡萄糖、半乳糖,其摩尔比为1.327:2.525:0.140:3.641:1.765;AFM观测表明S-ACP为多侧支的雪花状结构,J-ACP为少侧支的简单环状结构,且二者均非单链存在;抗氧化活性结果表明S-ACP活性高于J-ACP。

A polysaccharide named S- ACP was extracted from Agrocybe aegerita by hot water, then a polysaccharide named J-ACP was isolated from the dried leaf mustard with alkali.The content of the protein and the total sugars were determined by Coomassic brilliant blue G-250 method and phenol-sulfuric acid method.The monosaccharide compositions of polysaccharides were analyzed by gas chromatography（ GC）.The structure of polysaccharides were investigated by atomic force microscopy（AFM）.Meanwhile,the antioxidant activity in vitro of polysaccharides were also tested.Conclusion. Xyl, Man, Glu and Gal were determined to be the components of S-ACP in the mol ratios of 2.527 ： 0.140 ： 1.284 ： 1.738. Rha, Xyl, Man, Glu and Gal were determined to be the components of J-ACP in the mol ratios of 1.327：2.525：0.140：3.641：1.765.1t was found that S-ACP on mica formed Multi-lateral Snowflake-like structure by AFM,while J-ACP existed in the form of simple ring-like structure.The results showed that S-ACP had stronger antioxidant activity than J-ACP.