酸奶中污染酵母菌和霉菌的分离及鉴定

The isolation and identification of yeast and molds from the spoilage yogurts

以室温放置直至变质的市售酸奶为样品,从中分离出2株酵母菌和4株霉菌,分别命名为Y1、Y2、M1、M2、M3、M4。酵母菌采用形态学和26SrDNAD1/D2区序列分析鉴定,确定Y1为乳酸克鲁维酵母(Kluyveromyces lactis),Y2为马克思克鲁维酵母(Kluyveromyces marxianus)。霉菌经菌落及菌体形态学鉴定,确定M1、M2、M4为青霉(Penicillum),M3为白地霉(Geotrichum candidum)。

Two yeast strains （ marked Y1, Y2） and 4 molds strains （ marked M1, M2, M3, M4） were isolated from spoilage yogurts which were purchased from local market. The yeast strains were identified by observing its morphology and 26S rDNA D1/D2 region sequence analysis,the results showed that Y1 was Kluyveromyces lactis, Y2 was Kluyveromyces marxianus.The molds strains were identified by colony and cell morphology,and M1, M2, M4.were Penicillum,M3 was Geotrichum candidum.