大豆多糖对S180荷瘤小鼠抗肿瘤作用的免疫机制研究

Effects of soybean polysaccharide on antitumor and immunological mechanism of mice bearing S_(180)

从免疫学角度出发探讨大豆多糖的抗肿瘤作用。采用动物移植性肿瘤实验观察大豆多糖对小鼠体内肿瘤细胞生长和免疫器官胸腺、脾脏的影响;MTT法测定T淋巴细胞增殖功能;流式细胞仪检测大豆多糖对S180荷瘤小鼠T淋巴细胞亚群的影响;ELISA法检测荷瘤小鼠血清中细胞因子IL-2含量。结果表明大豆多糖对S180肉瘤生长有抑制作用,且以高剂量最佳;各剂量组对脾脏指数均有显著性提高,但中、高剂量给药组对胸腺有抑制作用;能提高小鼠脾淋巴细胞的增殖活性,与阴性对照组相比,中、高剂量组差异显著。大豆多糖明显增强ConA刺激的淋巴细胞增殖,与阴性对照组比较,中、高剂量组差异显著;能够不同程度地提高S180荷瘤小鼠脾细胞CD4+细胞数,增加CD4+/CD8+细胞比值;能提高S180荷瘤小鼠外周血血清中IL-2的含量。大豆多糖通过提高T淋巴细胞的增殖活性,并促进IL-2等细胞因子的分泌,由此发挥抗肿瘤作用。

To study the effect of soybean polysaccharides （SSPS）on antitumor and immune regulation of mice bearing S180. The effect of SSPS on antitumor and spleen index and thymus index were observed by tumor transplant experiment of animal; MTT examines T-lymphoid cell proliferation of the mice of tumor cell with S180; Adopting flow cytometry to determine T cell faciation;Use the ELISA method to examine the serum level of IL-2 in tumor mice.SSPS can inhibit the growth of S180 and the effect of high dose group was best.The spleen index was influenced by every dose group but thymus index was inhibited by middle and high dose;it can enhance the T cell proliferation after the ConA provok and has significantly difference.SSPS can enhance the CD4＋ T cell number in different extent, increase the CD4＋/CD8＋ cell ratio; SSPS can obviously enhance the serum level of IL-2, have significantly difference.SSPS had the notable anti-tumor, increased T-lymphoid cell proliferation, and promoted IL-2 secreting thus played its anti-tumor role.