海洋胶原肽对窒息仔鼠神经认知功能改善作用

Effect of marine collagen peptides on neurocognitive function in asphyxial newborn rats

目的探讨海洋胶原肽对窒息仔鼠近期及远期神经认知功能影响。方法 70只健康成年SD大鼠交配受孕,孕鼠于临产前1 d行延迟剖宫产术,建立新生仔鼠窒息模型;窒息仔鼠随机分为窒息组和海洋胶原肽低(0.225%)、中(0.45%)、高(1.35%)剂量组,正常分娩仔鼠为对照组;观察仔鼠出生后生理发育和神经反射达标情况,并在断乳时和3月龄分别进行Morris水迷宫实验。结果窒息组和3个MCPs干预组生理和神经反射指标达标时间均长于对照组(P<0.05);Morris水迷宫实验结果显示,断乳时窒息组和3个MCPs干预组逃避潜伏期较对照组延长(P<0.05);对照组、窒息组、低、中、高MCPs组穿越平台次数分别为(7.00±1.706)、(3.92±1.505)、(4.33±1.497)、(4.50±1.931)、(4.67±1.371),差异有统计学意义(P<0.05);3月龄时,与窒息组比较,3个MCPs干预组大鼠逃避潜伏期明显缩短(P<0.01)、穿越平台次数明显增多(P<0.05)。结论一定剂量的海洋胶原肽能够改善窒息仔鼠远期学习记忆能力。

Objective To investigate the effect of marine collagen peptides （MCPs） on short-term and long-term neurocognitive function in asphyxia/newborn SD rats. Methods 70 healthy adult SD rats were selected and mated. Asphyx- ia model was established by ＂delayed cesarean section＂ within the last day of gestation. Then asphyxia/ newborn SD rats were randomly divided into a asphyxia/group and the low （0. 225 % ）, moderate（ 0. 45 % ）, and high （ 1.35 % ） MCPs inter- vention groups. The normal labor newborn rats were used as the control group. The physiological and neural development index of the newborn rats were determined and Morris water maze test were performed for 21-day old and three-month old rats, respectively. Results The time to expected physiological and neural development index of the asphyxia/ group and three MCPs intervention groups were significantly longer than that of the control group （ P 〈 0.05 ）. Morris water maze test indicated that,at the time of 21 day, compared with the control group, the asphyxial group and three MCPs intevention groups had longer escape latency （ P 〈 0. 05 ）. The number of platform crossing of the control group, the asphyxia/group, the low,moderate and high MCPs groups were 7. 00 ± 1. 706,3.92 ± 1. 505,4. 33 ± 1. 497,4. 50 ± 1. 931, and 4. 67 ± 1. 371, with significant differences （ P 〈 0.05 ）. At the time of three-month, the escape latency of the three MCPs intervention groups were significantly shorter than that of the asphyxia/group（P 〈 0.01 ） and the times of platform crossing were significantly more than that of the asphyxia/group （ P 〈 0. 05 ）. Conclusion Certain amount of MCPs could improve the long-term learn- ing and memory ability of asphyxia/newborn rats.