DHA对老年大鼠嗅球神经因子和载脂蛋白影响

Effects of DHA on NGF,BDNF,APOE,APOJ in olfactory bulb of aged rat

目的探讨二十二碳六烯酸(DHA)对老年大鼠嗅球中神经生长因子(NGF)和脑源性神经营养因子(BD-NF)和载脂蛋白E(APOE)和J(APOJ)的影响。方法分别用180、360 mg/kg DHA灌胃24月龄老年大鼠49 d,处死大鼠取嗅球,用酶联免疫吸附试验(ELISA)测定脑组织NGF、BDNF含量,用western blotting测定载脂蛋白E和J的表达量。结果 180、360 mg/kg DHA组NGF分别为(11.17±1.27)、(12.48±2.35)pg/mg,与老年对照组(8.21±1.17)pg/mg比较,分别提高了36.05%和52.01%;180、360 mg/kg DHA组BDNF分别为(28.23±2.74)、(30.76±2.71)pg/mg,与老年对照组比较,分别提高了17.45%与28.01%;同时DHA组还增加老年大鼠嗅球的载脂蛋白APOJ含量,降低APOE的含量。结论二十二碳六烯酸对老年大鼠嗅球有保护作用。

Objective To explore the effects of docosahexaenoic acid（DHA） on neurotrophic growth factors （NFG）, brain derived neurotrophic factor（ B DNF）, apolipoprotein E（ APOE）, and apolipoprotein J（APOJ） in olfactory bulb of aged rat. Methods Sprague-Dawley （SD） rats of 24 months were treated with 180,360 mg/kg, d of DHA via oral gavage for 49 days. The rats were killed and the olfactory bulb was collected. NGF, BDNF levels were determined with enzym-linked immunosorbent assay（ELISA） and western blotting method was used to assay APOE and APOJ protein levels. Results DHA supplementation increased the levels of NGF（ 11.17 ± 1.27 pg/mg for 180 mg/kg DHA group,12. 48 ±2. 35 pg/mg for 360 mg/kg DHA group） with the increases of 36. 05 % and 52.01%, respectively, compared with those of the aged group （8.21 ± 1.17 pg/mg）. The levels of BDNF（28.23 ±2.74 pg/mg for 180 mg/kg DHA group,30.76 ±2. 71 pg/mg for 360 mg/kg DHA group）increased by 17.45% and 28. 01%, respectively, compared with those of the aged rat. DHA also increased APOJ protein level and decreased APOE protein level in olfactory bulb of the aged rat, compared with those of the control group significantly. Conclusion Docosahexaenoic acid has a protective effect on olfactory bulb of aged rat.