反相高效液相色谱法拆分西酞普兰对映异构体

RP-HPLC chiral separation of citalopram

目的:建立西酞普兰对映异构体的HPLC手性分析方法,用于草酸艾司西酞普兰光学纯度的分析测定。方法:采用不易变性的卵类粘蛋白键合硅胶(ES-OVM)为手性固定相,以乙腈-0.05 mol.L-1磷酸盐缓冲液(pH 7.0)(15︰85)为流动相,流速为1.0 mL.min-1,柱温30℃,检测波长240 nm,对西酞普兰对映异构体进行分离测定。结果:西酞普兰对映异构体在卵粘蛋白柱上实现了良好的分离,分离度为1.90。将该方法应用于手性拆分得到的草酸艾司西酞普兰的光学纯度测定,结果光学纯度大于97.9%。结论:该方法分离度与重现性好,可用于西酞普兰的光学纯度的分析测定及对拆分过程进行监测,结果可靠。

Objective ：To establish an HPLC method to separate citalopram enantiomers on ovomucoid chiral column for optical purity detection of escitalopram oxalate. Methods： The mobile phase was recommended as acetonitrile -0. 05 mol ·L^-1 phosphate buffer（pH 7.0） （15：85）at a flow rate of 1.0 mL · min^-1. The column temperature was 30 ℃ and the detection wavelength was 240 nm. Results： Citalopram enantiomers were separated under the above conditions, the resolution between S - and R - citalopram was 1.90. The optical purity of escitalopram ox- alate was more than 97.9%. Conclusion： With good resolution and repeatability,the method can be reliably used for chiral separation of citalopram enantiomers, and determination of the optical purity of S - and R - citalopram sample.