摘要
目的:观察补肾填精方对Aβ1-40所致老年性痴呆模型大鼠行为学及病理学影响。方法:84只SD大鼠随机分为空白组,模型组,补肾填精方小剂量组、中剂量组、大剂量组,双益平组。采用大鼠双侧海马CA1区注射Aβ1-40复制老年性痴呆模型。造模24h后开始连续给药3周。Morris水迷宫法测试大鼠学习记忆能力,透射射电镜观察大鼠海马CA1区神经元、突触的变化。结果:补肾填精方可改善大鼠的学习记忆能力、改善海马CA1区神经元结构、减少突触的丧失。结论:补肾填精方能改善Aβ1-40所致老年性痴呆模型大鼠行为学及脑组织的病理损害。
Objective: To observe the effects of nourishing kidney-essence formula on the behavior and pathological changes in senile dementia rats induced by Aβ1-40.Methods:84 SD rats were randomly divided into normal group,model group,low-dose,middle dose and high-dose of nourishing kidney-essence formula group,and Huperzine A group.Senile dementia was induced by Aβ1-40 injection in bilateral hippocampal CA1 area of these rats.After 24h of Aβ1-40 injection,drugs were continuously given for 3 weeks.Learning and remembering abilities were observed by water maze test,neurons and synaptic changes in hippocampal CA1 were observed by transmission electronic microscopy.Results: Learning and remembering abilities of senile dementia rats were significantly improved by nourishing kidney-essence formula treatment,especially in middle dose of nourishing kidney-essence formula.Nourishing kidney-essence formula also showed the effects of improving structure of hippocampal CA1 neurons,and reducing synapse loss.Conclusion: Nourishing kidney-essence formula could improve the behavior and pathological damage in hippocampal CA1 in Aβ1-40 induced senile dementia Rats.
出处
《中华中医药学刊》
CAS
2012年第1期23-26,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81072789)
国家基础研究发展计划(973计划)资助项目(2010CB530400)
上海市教委创新项目(10YZ58)
上海市科委中药现代化项目(10DZ1971200)
关键词
中医药
补肾填精方
老年性痴呆
学习记忆
海马神经元
Traditional Chinese Medicine
Nourishing kidney-essence formula
Senile dementia
Learning and memory
Hippocampal neuron