补肾填精方对Aβ_(1-40)所致老年性痴呆模型大鼠行为学及病理学的影响

Effects of Nourishing Kidney-essence Formula on Behavior And Pathological Changes in Aβ_(1-40) Induced Senile Dementia Rats

目的:观察补肾填精方对Aβ1-40所致老年性痴呆模型大鼠行为学及病理学影响。方法:84只SD大鼠随机分为空白组,模型组,补肾填精方小剂量组、中剂量组、大剂量组,双益平组。采用大鼠双侧海马CA1区注射Aβ1-40复制老年性痴呆模型。造模24h后开始连续给药3周。Morris水迷宫法测试大鼠学习记忆能力,透射射电镜观察大鼠海马CA1区神经元、突触的变化。结果:补肾填精方可改善大鼠的学习记忆能力、改善海马CA1区神经元结构、减少突触的丧失。结论:补肾填精方能改善Aβ1-40所致老年性痴呆模型大鼠行为学及脑组织的病理损害。

Objective： To observe the effects of nourishing kidney-essence formula on the behavior and pathological changes in senile dementia rats induced by Aβ1-40.Methods：84 SD rats were randomly divided into normal group,model group,low-dose,middle dose and high-dose of nourishing kidney-essence formula group,and Huperzine A group.Senile dementia was induced by Aβ1-40 injection in bilateral hippocampal CA1 area of these rats.After 24h of Aβ1-40 injection,drugs were continuously given for 3 weeks.Learning and remembering abilities were observed by water maze test,neurons and synaptic changes in hippocampal CA1 were observed by transmission electronic microscopy.Results： Learning and remembering abilities of senile dementia rats were significantly improved by nourishing kidney-essence formula treatment,especially in middle dose of nourishing kidney-essence formula.Nourishing kidney-essence formula also showed the effects of improving structure of hippocampal CA1 neurons,and reducing synapse loss.Conclusion： Nourishing kidney-essence formula could improve the behavior and pathological damage in hippocampal CA1 in Aβ1-40 induced senile dementia Rats.