摘要
目的:建立保健食品中非法添加的18种止咳平喘化学成分的快速筛选方法。方法:采用液相色谱-串联质谱法(LC-MS/MS)以Polaris 5 C18-A(2.0mm×50mm,5μm)为分析柱,流动相采用0.1%甲酸和甲醇进行梯度洗脱,电喷雾电离(ESI)、多反应监测(MRM)扫描方式,对保健食品中非法添加的18种止咳平喘化学成分进行筛选分析。结果:建立了快速分离检测保健食品中18种非法添加成分的测定方法,检测限低于2ng。结论:该方法专属性强、灵敏度高,适用于止咳平喘类保健品中非法添加成分的筛选及确证工作。
Objective：To establish a liquid chromatography-tandem mass chromatography（LC-MS/MS） method for the simultaneous screening 18 kinds of cough-relieving cough asthma chemical compositions in health food.Methods：This method involves liquid chromatography-tandem mass spectrometry,the separation was conducted by Varian Polaris 5 C18-A（2.0mm×50mm,5μm）.The mobile phase was consisted of 0.1% formic and methanol.The target compounds were analyzed with LC-MS/MS-ESI,multiple-reaction monitoring（MRM） was used to screen illegal chemical compositions in health food.Results：A fast and sensitive liquid chromatography mass spectrometric（LC/MS） method for the simultaneous screening for illegal chemical compositions was described.The LOD of these substances were below 2ng.Conclusion：The method was sufficiently selective and sensitive to detect illegal chemical compositions in health food.
出处
《中华中医药学刊》
CAS
2012年第1期172-175,共4页
Chinese Archives of Traditional Chinese Medicine
基金
南京市科技发展计划项目(200801069)
