高肺血流幼猪模型的建立及其肺动脉血流动力学和肺组织学变化

Impact of high pulmonary blood flow on hemodynamics and pulmonary artery organizations in piglet model

目的研究高肺血流幼猪动物模型的建立的方法及探讨肺动脉血流动力学与结构关系。方法采用生后2-3月龄的幼猪,体重8-10kg,分为两组,每组6只。对照组(Control-group,n=6):即假手术组,只开胸无手术处理;高肺血流组(shunt-group,n=6):全部行左侧开胸,降主动脉与左肺动脉搭桥术。所有动物正常饲养3个月。然后正中开胸,检测肺动脉收缩压(PASP)、肺血管阻力(PVR),观察肺动脉标本的结构和组织学变化。结果 高肺血流组肺动脉收缩压(PASP)、肺血管阻力(PVR)显著升高(P<0.01);左肺动脉出现平滑肌细胞显著增生,部分血管中膜及内膜增厚,官腔狭窄。对照组无明显变化。结论在幼猪动物模型中,高肺血流可导致肺动脉结构重塑,可导致肺动脉压力的升高。为进一步研究高肺血流先天性心脏病肺动脉高压的形成过程,以期在治疗上提供新的方法。

Objective To research the methods of the high pulmonary blood piglet models established and relation of pulmonary artery pressure and structure. Methods Piglets the age of 2--3 months and weighs 8--10 kg were divided into two groups randomly,each group of six. the only Control group（n = 6）, or sham group, only that have not been dealt with the operation of the lungs set;High pulmonary blood group （shunt group, six） ：all the line left open, cut to the left pulmonary artery bypass surgery. All animals are normally raised three months, then the open,and testing pulmonary arterial systolic pressure （PASP）and pulmonary vascular resistance（PVR）,observing the organization of pulmonary artery. Results In shunt group,pulmonary arterial systolic pressure （PASP）and pulmonary vascular resistance （ PVR ） significantly increase（P〈0. 01） ;Smooth muscle cell in pulmonary artery shown in remarkably,in part of the tunicae intima vasorum thickend. In Control group,no apparent change of pulmonary arterial systolic pressure and pulmonary vascular resistance. Conclusion In piglet models, high lung blood flow could induce the reshaping of the pulmonary artery structure, could lead to pulmonary hypertension. To further study the mechanism of pulmonary hypertension induced by high pulmonary blood flow provides new methods in the treatment.