内皮细胞中剪切型XBP1的过表达对动脉粥样硬化形成的影响

The impact of excessive expression of spliced XBP1 in endothelial cells on atherosclerosis

目的探讨血管壁内皮细胞中剪切型XBP1的过表达对动脉粥样硬化形成的影响。方法从产后的健康新生儿脐带获取脐静脉内皮细胞,脐静脉内皮细胞传代至第3代后,以不同浓度(10mg/L,20mg/L)的氧化胆固醇(7-酮基胆固醇,7-KC)温育脐静脉内皮细胞,应用Western Blot的方法,检测脐静脉内皮细胞中剪切型XBP1的蛋白表达水平,凋亡蛋白Caspase3的活性,应用流式细胞学的方法检测脐静脉内皮细胞的凋亡率和继发性细胞坏死阳性率。结果 随着7-KC温育浓度的增加,脐静脉内皮细胞中剪切型XBP1的蛋白表达水平增加,Caspase3的活性增加,脐静脉内皮细胞的凋亡率和继发性细胞坏死阳性率增加。结论血管壁内皮细胞S-XBP1的表达可能会激活凋亡途径,使内皮细胞发生凋亡,启动AS的形成。

Objective To study the impact of excessive expression of S-XBP1 in vascular endothelial cells on athero sclerosis. Methods Human umbilical vein endothelial cells （HUVECs） were isolated from postnatal human umbilical vein,were up to passage 3 and incubated with different concentration of oxidative cholesterol, 7-ketocholesterol （7- KC）. The S-XBP1 protein in cells were detected by Western Blot, the caspase3 activity in cells was examined,the apop tosis ratio and positive ratio of insecondary dead cells were detected by Flow Cytometry. Results In cells incubated with 7-KC,S-XBP1 protein were up-regulated, the caspase3 activity was up-regulated, the apoptosis ratio and positive ratio of insecondary dead cells were up regulated, and all of which were dependent on the concentration increase. Conclusion S-XBP1 expression in vascular endothelial cells may activate apoptosis pathway,make vascular endothelial cells apoptotic,and lead to atherosclerosis development.