摘要
合成了一种具有双光子荧光探针功能的有机纳米粒子2,5,2',5'-(4'-N,N-二苯胺苯乙烯基)联苯(DPA-TSB),并研究其细胞毒性.利用水溶性四氮唑(WST-1)法、乳酸脱氮酶(LDH)法和流式细胞术检测了胃癌细胞吞噬纳米粒子后的生理活性.研究结果表明,在纳米粒子浓度小于12μg/mL时,胃癌细胞仍表现出较好的生理活性,表明该纳米粒子是一种具有较好生物安全性的光功能有机纳米粒子.
The cytotoxicity about organic nanoparticles which has the function of two-photon fluorescent probe 2,5,2′,5′-tetra(4′-N,N-diphenylaminostyryl)biphenyl(DPA-TSB) was studied.The physiological activity of stomach cancer cells after englobing the nanoparticle was detected by the methods of WAT-1,Lactate dehydrogenase(LDH) and flow cytometry.The results show that the stomach cancer cells can live well when the concentration of the nanoparticles is below 12 μg/mL,which suggests that the nanoparticles have the property of good biological security.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2012年第1期210-214,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:51173066)资助