小儿消咳片中白屈菜红碱的含量测定

Content Determination of Toddaline in Xiao'er Xiaoke Tablets by HPLC

目的建立高效液相色谱(HPLC)法测定小儿消咳片中白屈菜红碱的含量测定方法。方法采用HPLC法,色谱柱:Zorbax SB-C18(250 mm×4.6 mm,5μm),流动相:乙腈-1%三乙胺溶液(磷酸调pH为3.0)(25∶75),检测波长:269 nm;流速1.0 mL.min-1,进样量10μL。结果白屈菜红碱在8.25～825.00μg.mL-1范围内线性关系良好(r=0.999 8),平均加样回收率为96.80%,RSD为1.11%(n=6)。结论该方法简便快捷,准确,重复性好,灵敏度高,可用于小儿消咳片的质量控制。

Objective To establish a HPLC method for determination of toddaline in xiao＇ er xiaoke bablets. Methods Zorbax SB-C18 （250 mm ×4.6 mm, 5 μm） was used. The mobile phase was aeetonitrile -1% triethylamine （ pH 3.0 adjusted by phosphoric acid） （22 ： 78）. The flow rate was 1.0 mL·min-1 and detection wavelength was set at 269 nm. Results The linear range of gastrodin was 8.25-825.00 μg ·min-1 （ r = 0. 999 8 ）. The average recovery was 96.80% （ n = 6 ） （ RSD = 1.11% ）. Conclusion This method is simple, accurate and reproductive,which can be used for the quality control of xiao＇ er xiaoke bablets.