质壁分离剂盐法提取啤酒酵母RNA

Extracting RNA from Yeast by the Combination of Salination and Plasmolyticum

在盐法破壁的基础上,添加质壁分离剂。当酵母浓度为10%、氯化钠浓度为5%时,添加5 mL乙酸乙酯,并在100℃下抽提2 h,可以增加核酸的提取率和纯度,使得提取率达到60%。

The plasmolyticum was added based on the salination.When the concentration of the yeast was 10% and salini- ty was 5 % ,5 mL ethyl acetate was added, the yeast was heated for 2 h at 100 ℃.In this way, the yield and the purity of nucleic acid could be increased and the yield could reach about 60 %.