咪唑类离子液体作流动相添加剂对苦参类生物碱保留行为的表征

The Representation of Imidazolium Ionic Liquids as Mobile Phase Additives on Matrine Alkaloids' Retention Behavior

目的明确离子液体作流动相添加剂高效液相法分离生物碱的过程和作用机理。方法利用离子液体作流动相添加剂,高效液相法分离苦参类生物碱,并根据2个经典的保留模型:溶质计量置换保留模型(SDM-R)和Snyder经验式,建立模型,考察离子液体浓度,烷基链长度和有机溶剂体积比对组分保留行为的影响,通过模型参数研究保留过程和保留机理。结果当离子液体作流动相添加剂,并达到一定浓度时,离子液体的浓度与组分的保留值符合SDM-R模型的线性关系,且Z值随离子液体烷基链长度的增加而减小,logI值增大。结论离子液体作为流动相添加剂时,生物碱的分离保留过程以竞争吸附作用为主,且符合SDM-R模型。

OBJECTIVE To clear the process and mechanism of using high performance liquid chromatography to separate alkaloids （ionic liquids as mobile phase additives）. METHODS This paper used high performance liquid chromatography to separate matrine alkaloids, and ionic liquids as mobile phase additives, built models according to two typical retention models： stoichiometric displacement model for retention of solute （SDM-R） and Snyder empirical, investigated the effect of ionic liquid concentration, alkyl chain length and volume ratio of organic solvent on retention behavior of components, and studied retention process and mechanism by model parameters. RESULTS When the ionic liquids reached a certain concentration as mobile phase additives, the concentration of ionic liquid and retention value of components comply with the linear relationship of SDM-R model, and Z values decreased with the increase of ionic liquid alkyl chain length while log/ value increased. CONCLUSION Separated retention process of alkaloids is dominated by competitive adsorption function and in accordance with SDM-R model when ionic liquids are as mobile phase additives.