中药硇砂不同品种抗炎作用及急性毒性实验研究

Research on Anti-inflammation and Acute Toxicity Test in Sal Ammoniac and Halite Violaceous

目的探讨硇砂不同品种的抗炎药理作用和安全性。方法采用二甲苯致小鼠耳肿胀法和小鼠腋窝皮下置无菌棉球致炎法,观察白硇砂、紫硇砂对急性炎症、亚急性炎症的对抗作用;采用小鼠体内急性毒理实验,评价白硇砂、紫硇砂的急性毒性。结果紫硇砂对二甲苯所致小鼠耳廓肿胀的抑制率为27.3%,对小鼠棉球肉芽肿组织增生的抑制率为20.0%,与生理盐水组相比有显著性差异(P<0.05)。白硇砂对小鼠耳廓肿胀及棉球肉芽肿的作用与生理盐水组相比,均无显著性差异。小鼠灌胃白硇砂的LD50为2.94g/kg,灌胃紫硇砂的LD50为4.82g/kg。结论紫硇砂对急性、亚急性炎症动物模型有对抗作用,而白硇砂作用不明显;硇砂各品种使用药典推荐剂量安全性较好,大幅度增加剂量后可产生毒性。

OBJECTIVE To study the anti-inflammatory effects and safety of Sal Ammoniac and Halite Violaceous. METHODS The anti inflammatory action of Sal Ammoniac and Halite Violaceous on acute inflammation and subacute inflammation was studied by mouse auricular swelling test induced by xylene and mouse inflammation model induced by planted cotton ball in axillary. The acute toxicity of Sal Ammoniac and Halite Violaceous was evaluated by acute toxicity experiment in rats. RESULTS Halite Violaceous raw material could inhibit the mouse ear edema induced by xylene with the inhibition rate of 27. 3%, and it could also decrease the cotton hall granuloma hyperplasia with the inhibition rate of 20.0%, which have significant difference with NS group（P〈0.05）. However, there was no significant difference among groups of Sal Ammoniac and NS group. The LD50 of Sal Ammoniac raw material was 2.94 g/kg ; which of Halite Violaceous was 4.82 g/kg. CONCLUSION These results suggest that only Halite Violaceous showed anti-inflammatory effects on both acute and subacute animal models, but Sal Ammoniac has no obvious effect. It is safe to use Sal Ammoniac and Halite Violaceous by oral administration with normal clinical dosage, but it can produce toxicity if increasing dose greatly.