Biolog-Eco解析黄山风景区空气微生物碳代谢多样性特征

Analysis of Carbon Metabolism Diversity Characters of Air Microbes in Huangshan Scenic Spot Using Biolog-Eco Method

采用Biolog生态微平板分析黄山风景区6个景区空气样品的微生物碳代谢群落结构,旨在了解不同景区空气微生物碳代谢功能群落结构的特点与差异.结果表明:6个景区AWCD值变化趋势不同,空气微生物在碳源利用能力、微生物丰度等方面存在差异,可能与海拔、气象、游客集中与流动等因素有关;6个景区空气微生物对6类31种碳源的利用程度存在差异,但总体对羧酸类和碳水化合物类的利用程度较高,对其它化合物类的利用程度较低;主成分分析显示,6个景区空气微生物代谢基质主成分1的贡献度为58.7%,主成分2为28.5%;主成分1荷载0.55以上的基质有25种,主成分2只有7种.Biolog生态微平板技术能够客观、正确表征空气微生物碳代谢多样性的特征,是研究空气微生物群落功能多样性的较理想方法之一.

The community structure of the air microbes in Huangshan Scenic Spot was measured by the Biolog Eco plate to study the characters and differences of the carbon catabolic diversity of air microbes in different spots. The results showed the change patterns of the AWCD （average well color development）in six scenic spots were different, mainly demonstrating in how the air microbes made use of the carbon sources and the abundance of the microbes. It may attribute to the height, the climate and the number of tourists, et al. Though the degree to which the air microbes in six scenic spots made use of 31 carbon sources of 6 types was different,in general, they made more use of the carboxylic acids and carbohydrates while making less use of other compounds. The PCA（principal component analysis）showed the contribution of the PC1 of the air microbes metabolism substrates in six scenic spots was 58.7% ,PC2 was 28.5% ,and there were 25 types of substrates in PC1 over 0.55 of load, only 7 in PC2. The technology of the Biolog Eco plate is one of the effective approaches to study the diversity of air microbes as it can show the characters of the carbon catabolic diversity of air microbes objectively and accurately.