香加皮不同组分多次给药对小鼠肝毒性损伤作用研究

The Research of Hepatotoxicity Damage Caused by Different Components from Cortex Periplocae in Mice

目的比较香加皮水提组分和醇提组分多次给药对小鼠肝毒性损伤的作用程度。方法给小鼠灌胃香加皮水提和醇提组分,每天1次,连续灌胃7天,按等生药量算,香加皮水提组分高、中、低剂量组分别为15.0,3.12,0.78 g.kg-1,醇提组分高、中、低剂量组分别为6.0,3.12,0.78 g.kg-1;观察小鼠一般状况,7天后检测肝毒性相关指标,检测血浆清蛋白(ALB)、血清碱性磷酸酶(ALP)、胆红素(TBIL)水平和肝组织病理检查。结果香加皮不同组分可导致血和肝内丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)活性增高,肝脏重量和肝体比值增大,血清碱性磷酸酶(ALP)、胆红素(TBIL)水平增加,血浆清蛋白(ALB)降低;肝组织病理可见肝细胞可见水肿、脂肪变性、灶性坏死、片状坏死;上述变化随剂量的增加而逐渐加重,呈现一定的剂量依赖性。肝毒性作用程度醇提组分>水提组分。结论较高剂量多次给予香加皮水提组分或醇提组分均可致小鼠明显的肝毒性损伤,即可致肝功能指标的改变,又可致肝细胞器质性病变。

Objective To investigate the hepatotoxicic effect induced by different components from Cortex Periplocae in mice. Methods Mice were administrated with water extracted and alcohol extracted components for 7 days. The general situation of mice were observed, hepatical toxicity related index and the level of ALB,ALP,TBIL in serum were detected, the hepatic tissue were examined. Results The water extracted and alcohol extracted components can cause the activity of ALT,AST in serum and hepatic tissue increased, as well as the gain in liver weight. The level of ALP, TBIL corresponding increased, however the level of ALB was reduced. All changes were aggravated in accordance with the dosages. The degree of hepatotoxicity damage caused by the alcohol extracted composition is more serious than that by the water extracted composition. Conclusion The continues administration of different components from Cortex Periplocae to nice can induce obvious hepato-toxicity injury, it not only cause the changes of liver function index, but the structural patho-logical changes of hepatic cell.