香加皮不同组分致小鼠肝毒性与氧化损伤相关性研究

The Research of Oxidative Damage Mechanism in Hepatical Toxical Injury Caused by Different Components from Cortex Periplocae in Mice

目的观察连续给予香加皮水提组分和醇提组分导致小鼠肝毒性损伤程度与氧化损伤的相关性。方法给小鼠灌胃香加皮水提组分和醇提组分,按含生药量计算香加皮水提组分高、中、低剂量分别为9.0,4.68,1.17 g.kg-1,醇提组分高、中、低剂量组分别为1.25,0.50,0.13 ml.kg-1连续给药7天,观察小鼠一般状况,检测肝功相关指标,血和肝组织内MDA含量、SOD活性、一氧化氮(NO)、一氧化氮合酶(NOS)、谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-Px)的含量和活性。结果香加皮水提组分和醇提组分均可致血中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性增高,血中和肝组织内MDA含量增加,同时SOD活性下降;血和肝组织中一氧化氮(NO)含量增加,一氧化氮合酶(NOS)活性升高;血和肝组织中谷胱甘肽(GSH)含量下降,谷胱甘肽过氧化物酶(GSH-Px)活性下降。上述变化趋势随剂量增加而加重,与空白对照组有明显差异。结论香加皮水提组分和醇提组分多次给药后可导致小鼠肝毒性损伤,其损伤途径与引起机体氧化应激后诱导脂质过氧化有关,且醇提组分的肝毒性损伤程度高于水提组分。

Objective To investigate the relationship of oxidative damage mechanism in hepatical toxical injury caused by different components from Cortex Periplocae in mice. Methods Mice were administrated with water extracted and alcohol extracted components repeatedly for 7 days. The general conditions of mice were observed, and the level of MDA.SOD^NO.NOS.GSH.GSH-Px in serum and hepatic tissue were detected, as well as the activity of ALT, AST in serum. Results The different components can cause the activity of MDA in serum and liver increased, the level of SOD corresponding induced, however the level of NO and NOS were increased. In addition the activity of GSH and GSH-Px reduced. All the changes were aggravated in accordance with the dosages. Conclusion The continuous administration ~of different components from Cortex Periplocae to mice can induce obvious hepato-toxicity injury; the approach of hepatic damage is related with the peroxidative damage mechanism. The degree ofhepatotoxicity damage caused by the alcohol extracted composition is more serious than that by the water extracted composition.