ABO基因定型解决正反定型不符合标本3例

Using ABO Genotype to Solve the Abnormal Serological Blood Group on 3 Samples

目的建立PCR-SSP(sequence specific primer,SSP)法ABO基因定型技术用于血型工作中常见问题的解决。方法运用血型血清学方法、PCR-SSP法进行3例实际工作中较有代表性的正反定型不符合样本检测。结果血清学方法检出了3例正反定型不一致的样本。基因检测结果显示:样本1、3是AB基因型;样本2是OIVOIV基因型。此3例样本血清学检测结果相互矛盾,不能得出正确结论。PCR-SSP法检测结果清晰,可及时准确定型。结论血型基因定型技术较血型血清学方法更准确、可靠,是血型血清学技术的极好补充。

Objective To set up a PCR-SSP method to solve the common problems in laboratory.Method To detect three typical abnormal samples by serological and PCR-SSP methods respectively.Result Three abnormal samples were detected by serological method.The results of genotypes showed that both of sample 1 and 3 were AB genotypes.The sample 2 was OIVOIV genotype.The three serological results showed kind of contradiction while PCR-SSP had accurate results.Conclusion Compared with the serological method,PCR-SSP shows more accurate and reliable character,which greatly reinforces serological technique.