不同生长时期人参茎中几种氧化还原酶的活力变化研究

Study on Activity Changes of Oxidoreductases in Ginseng Stems in the Different Growth Stages

研究人参在生长季的不同时期茎中几种氧化还原酶的活力变化。以5年生人参茎为供试材料,采用中性磷酸缓冲溶液提取粗酶液。应用紫外分光光度法分别测定过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)和抗坏血酸过氧化物酶(APX)的活力。结果表明,在展叶期、开花期和结果期4种氧化还原酶活力都出现峰值,并以结果期最高;果后参根生长期之后4种酶活力处于较低水平,且变化都比较平缓。人参茎中4种氧化还原酶的活力变化与茎的生长,花和果实的形成密切相关。

The aim was to study the changes of oxidoreductase activity of ginseng stems in the different growth stages of growing season. A five-year old ginseng stem was used as material. Neutral phosphate buffer was selected to extract crude extract. UV was applied to determine the activities of peroxidase （POD）, catalase （CAT）, polyphenol oxidase （PPO）, ascorbate peroxidase （APX）. Activity peaks of four kinds of oxidoreductase appeared in the spreading stage, the flowering stage and the fruit growth stage, and the highest was in the fruit growth stage. 4 kinds of enzyme activity were at a low level, and changes of enzyme activity were relatively flat after the root growing after fruit stage. Changes of 4 kinds of enzyme activity in ginseng stem were closely related to stem growth, flower and fruit formation.