不同施氮水平下烤烟多酚含量与烤后烟叶化学指标关系研究

Study on the Relationship between Polyphenols Content and Chemical Index of Flue-cured Tobacco in Different Amount of Applied Nitrogen

通过田间试验和化学分析等方法,研究不同施氮量对‘翠碧1号’烤烟多酚含量的影响及其与烟叶化学成分之间的关系。结果表明,不同施氮水平下,绿原酸和芸香苷占5种多酚类物质总量的77.77%～88.64%;随施氮量上升,烤后烟叶的绿原酸、4-o-咖啡奎尼酸、芸香苷、莨菪亭含量和5种多酚总量均表现为先明显下降再略有上升的趋势,新绿原酸含量表现为上升的趋势。5种多酚类物质含量及其总量随烟叶总氮和烟碱含量、还原糖/烟碱比值和总氮/烟碱比值的变化均呈二元二次曲线变化,相关关系显著或极显著。

Field experiments and chemical analysis were carried out to study the effects of different nitrogen fertilizers on the polyphenols and there relationship with chemical composition of flue-cured tobacco leaves. The results showed that, the proportion of chlorogenic acid and rutin was in the majority （from 77.77% to 88.64% ） in different amount of applied nitrogen; as the increasing of the amount of applied nitrogen, chlorogenic acid content, 4-o-caffeoyl quinic acid content, rutin content, scopoletin content and the totals of 5 kinds of polyphenols were decreased markedly at first, and then increased slightly, while neochlorogenic acid content had the increasing trend. The relationship between polyphenols content （5 kinds of polyphenols and there totals） and main chemical composition （nicotine content, total nitrogen content, the ratio of reducing sugar to nicotine and total nitrogen to nicotine） could be simulated by dualistic and quadratic equation respectively with highly significant relation.