HPLC测定不同产地傣药肾茶中熊果酸的含量

Determination of Ursolic Acid in Dai Medicine- Orthosiphonari Status(Shencha) from Different Regions by HPLC

目的:建立HPLC测定傣药肾茶中熊果酸含量的方法,研究不同产地肾茶中熊果酸含量的差异。方法:色谱柱为EclipseXDB-C_(18)(250mm×4.6mm,5μm),流动相为甲醇-0.5%乙酸铵(88:12),体积流量1.0mL/min,柱温为25℃,检测波长210nm,图谱采集时间为20min。结果:熊果酸与其他成分分离良好,在0.14～1.40μg范围内线性关系良好,r=0.9996,平均回收率为99.82%,RSD为0.79%(n=6)。结论:该法简便、准确可行,可用于傣药肾茶中熊果酸的含量测定及肾茶的质量控制,不同产地熊果酸含量有较大差异。

Objective：To establish a HPLC method for the determination of ursolic acid in Dai Medicine - Orthosiphonari Status （Shencha）,and compare the contents of different regions.Methods：Analyses were performed on Eclipse XDB-C18（250 mm×4.6 mm, 5μm） column with methanol-0.5%ammonium acetate system（88：12） as mobile phase at a flow rate of 1.0ml·min^（-1）.The detection wave length was 210 run,column temperature was 25℃.Results：There is a good linearity between the amount range of 0.14～1.40μg（r= 0.9996）.The average recovery was 99.82%and the RSD was 0.79%（n=6）.Conclusion：The method is simple and accurate,which can be used to determine the contents of ursolic acid in Dai Medicine- Orthosiphonari Status（Shencha） and can be used for its quality control.The content of ursolic acid in Orthosiphonari Status from different regions is quite different.