基于拆方的蒙药地格达-4味汤配伍规律研究

Study on compatibility law of Mongolian prescription Digeda soap-4 by disassembled

目的:通过拆方的方法探讨蒙药"肋柱花-4味汤"配伍规律。方法:将蒙药"肋柱花-4味汤"拆成肋柱花、栀子、瞿麦和胡黄连等4个单味药,给急性肝损伤模型大鼠灌胃相应的药液后,在不同时间点取血,离心分离血清。以高效液相色谱(HPLC)法测定指标性成分獐牙菜苦苷、栀子苷、胡黄连苷Ⅱ含量;用分光光度法测定瞿麦总黄酮含量。根据四个单味药与地格达-4味汤的指标性成分含量变化,评价四个单味药在复方中"君、臣、佐、使"的关系。结果:肋柱花、栀子、瞿麦、和胡黄连组成方剂后,除了獐牙菜苦苷升高外其他三个成分均有降低趋势。结论:初步认为肋柱花为君药、栀子为臣药、胡黄连为臣药或佐药、瞿麦为使药。

Objectivr：To discuss the compatibility law of Digeda soap-4 by the method of disassembled.Methods：Digeda soap-4 was disassembled to Lomatogonium rotatum、Gardenia、Fringed pink、Picrorhiza four parts,then intragastric administration to rat model of acute liver injury.The blood samples were collected in different time and separated the serum by Centrifuge.A high-performance liquid chromatographic method was developed to determine the content of indicator components Swertiamarin、Geniposide、PicrosideⅡ.The method of Spectrophotometry was used to determine the Flavonoids content of Fringed pink.According to the changes of indicator components,content of four single herb and Digeda soap-4,we evaluated the relationships between ＂Jun、Chen、Zuo、Shi＂ of four single herb in prescription.Result：After Lomatogonium rotatum、Gardenia、Fringed pink、Picrorhiza formed a prescription,all three components were decreased,only Swertiamarin was increased.Conclution：Preliminary,we view Lomatogonium rotatum as ＂Jun＂、Gardenia as ＂ Chen＂、Picrorhiza as ＂zuo＂ or ＂Chen＂、Fringed pink as ＂Shi＂ respectively.