小鼠CD4^+T细胞活化后IL-10受体Ⅰ表达的研究

The study of the IL-10 receptor Ⅰ expression level on the mouse CD4^+ T cell after stimulation

目的:检测抗小鼠CD4+T淋巴细胞活化后表面白细胞介素10受体I(IL-10R1)表达变化,为进一步研究白细胞介素10(IL-10)相关免疫疾病的发病机制及进展提供有力支持。方法:取C57BL/6小鼠脾细胞,溶血后采用尼龙毛去除B淋巴细胞后,流式细胞仪无菌分选CD4+T淋巴细胞,利用anti-CD3和anti-CD28多克隆抗体刺激分选后细胞,分别在第0、12、24、48和72小时流式细胞术检测IL-10R1表达情况。结果:0小时时小鼠CD4+T淋巴细胞不表达IL-10R1,随着刺激时间的延长IL-10R1表达增加,24小时时达到最高,随后表达水平开始下降,72小时时表达情况与0小时基本相同。结论:CD4+T淋巴细胞活化后IL-10R1表达短暂,以24小时时表达最高。

Objective：To study the IL-10 receptor I（IL-10R1） expression level on the activated mouse CD4＋ T cell and provide strong supply for studying the pathogenesis and progress of IL-10 related autoimmune diseases.Methods：The spleen cells of C57BL/6 mouse were isolated and B cells was got rid of by nylon filter after hemolysis.CD4＋T cells were isolated sterilely by flow cytometry.The IL-10R1 level was detected on the CD4＋T cell at the 0th,12th,24th,48th and 72th hour by flow cytometry after stimulation by anti-CD3 and anti-CD28 polyclonal antibody.Results：At the 0th hour CD4＋T cell did not express IL-10R1.With the extension of time the level of IL-10R1 were increased and at the 24th hour reached the peak.Then the level of IL-10R1 began to decrease and at 72th hour the level of IL-10R1 was similar with the level at 0th hour.Conclusion：The expression of IL-10R1 on activated CD4＋T cell is short in mice,with peak in 24 hours.