日粮添加维生素E对免疫抑制产蛋鸡经济性状下降的调控效应

Modulation of Dietry Vitamin E on Economic Characterization of Immunosupressed Laying Hens

【目的】研究在基础日粮中添加维生素E对环磷酰胺诱导产蛋鸡免疫抑制的调控效应。【方法】将270只健康尼克褐产蛋鸡随机等分为5组,每组3个重复,每个重复18只。Ⅰ、Ⅱ组饲喂基础日粮(维生素E含量44.59 mg.kg-1),Ⅲ、Ⅳ、Ⅴ组在基础日粮中分别添加50、100和200 mg.kg-1维生素E。试验第5、6、7天,对Ⅱ—Ⅴ组产蛋鸡注射80 mg.kg-1 BW环磷酰胺建立免疫抑制模型,第Ⅰ组注射等量生理盐水。【结果】日粮中添加VE能显著提高免疫抑制产蛋鸡的产蛋性能和饲料转化率、养分表观代谢率(P<0.05),提高免疫抑制产蛋鸡脾脏和胸腺的相对重量和新城疫(new castle disease,ND)、H5和H9亚型禽流感(avian influenza,AI)抗体滴度及血浆谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)活性(P<0.05),降低丙二醛(malondialdehyde,MDA)的含量(P<0.05);提高免疫抑制产蛋鸡血浆的PGE2、IL-1、IL-6和TNF-α含量(P<0.05)。【结论】环磷酰胺诱导产蛋鸡所产生的免疫抑制可显著降低其生产性能、养分利用率、抗体水平、抗氧化功能及PGE2和炎性细胞因子含量;日粮中添加维生素E对免疫抑制蛋鸡具有明显的调控效应,且添加水平为50和100 mg.kg-1时效果优于200 mg.kg-1的添加量。

【Objective】The modulation of dietary vitamin E levels on immunosuppressed laying hens induced by cyclophosphamide（CTX） was studied.【Method】Two hundred and seventy Nick Red laying hens were randomly divided into 5 groups and each group had 3 replications with 18 birds.Laying hens from group Ⅲ,Ⅳ,and Ⅴ（birds treated with vitamin E） were fed diets supplemented with 50,100 and 200 mg·kg-1 vitamin E,respectively,whereas group Ⅰ and Ⅱ received only the corn-soybean meal basal diets with 44.59 mg·kg-1 of vitamin E.Laying hens in group Ⅱ-Ⅳ（birds challenged with CTX） were injected in the thigh muscle with CTX at 80 body weight for 3 days,and that in groupⅠ（control group） were injected with the same amount of sterile saline.【Result】Supplemental vitamin E significantly increased egg production,feed efficiency and nutrient digestibility（P0.05）.The relative weights of the spleen and thymus to the body weight,serum HI antibody titers to H5-AI,H9-AI and ND,plasma GSH-Px and SOD activities were greater in immunosuppressed birds fed supplemented vitamin E（P0.05）,and the MDA concentration was least at 50 mg·kg-1 supplemental vitamin E,and further additions had negative effect（P0.05）.Birds treated with CTX fed diets with supplemental 100 and 200 mg vitamin E/kg had greater plasma PGE2,IL-1,IL-6,and TNF-α levels（P0.05）.【Conclusion】The results showed that dietary vitamin E supplementation significantly attenuated immunosuppressive effect of CTX challenge in laying hens.