牛布鲁氏菌抗体ELISA试剂盒与国标血清学SAT试验在布病检测中的比较试验

A Comparison of Bovine Brucellosis ELISA Kit from Standard Tube Agglutination Test(SAT) in the Serological Investigation of Brucellosis in Xinjiang Region of China

采集的牛血清100份、羊血清250份,分别进行国标布病虎红平板凝集试验(RBT),在牛血清中检出13份布病阳性血清,羊均为阴性。对牛的13份阳性血清分别进行动物布病试管凝集试验(SAT)和牛布鲁氏菌抗体ELISA试验。经SAT试验结果为10份阳性,ELISA试验结果为13份阳性。结果表明牛布鲁氏菌抗体ELISA试剂盒快速,操作简便,敏感性好,特异性优待研究,如果能够证明特异性高,在布病血清学检测的有效的方法。

100 serum samples from cattle and 250 serum samples from sheep were detected by GB Bengal test （RBT）,the result showed that 13 positive in cattle,sheep almost obtain negative results. 13 positive serum samples from cattle differently detected by Standard tube agglutination test （SAT） and bovine brucellosis ELISA kit ,The tests showed that SAT detected 10 positive ,and ELISA kit detected 13 positive ,the date suggested that the bovine brucellosis ELISA kit was of high sensitivity and convenient fast operation ,the specificty needs futher tests,if it confirmed, provides an effective means for the serological test methods of brucellosis.