浓核病毒感染早期家蚕血液、中肠蛋白表达差异比较分析

Differential Analysis of Expressed Proteins in Midgut and Hemolymph of Silkworm at the Early Stage of Infection by Densovirus

为了探明在浓核病毒镇江株(BmDNV-ZJ)侵染早期,家蚕部分组织蛋白所产生的免疫抵抗性变化机制,本实验采用差异蛋白质组学技术研究分析了BmDNV-ZJ感染早期,家蚕的中肠、血液组织中特异性表达的差异蛋白。实验结果表明:在浓核病毒侵染初期,感受性家蚕的中肠组织受病毒感染而得到特异性表达的蛋白可能为丝氨酸蛋白酶抑制剂和巯基抗氧化酶蛋白,前者具有调控蛋白酶活性和细胞凋亡的功能,后者具有抗氧化的作用。血液组织受病毒感染诱导而产生的蛋白可能是丝裂原活化蛋白激酶和类抗氧化酶蛋白,前者具有调控细胞凋亡的功能,后者具有抗氧化、消除自由基作用。由于试验中所得的差异蛋白点很少,这表明在BmDNV-ZJ感染早期,蚕体对浓核病毒的感染而产生的反应很小。蚕体可通过被侵染的中肠组织(浓核病毒感染的靶部位)以及血液(免疫组织)共同产生一些抗氧化或调控细胞凋亡的酶蛋白等来抗击浓核病毒的侵染。

To understand the innate immune response of silkworm at the early stage of infection by Bombyx mori densovirus Zhenjiang strain （BmDNV-ZJ）, the differential proteomic technology was applied to analyzed the protein of midgut and hemolymph of the fourth instar larva of silkworm. The results showed that some differentially expressed proteins such as serpin-2 or thiolperoxiredoxin were checked out in midgut of silkworm at the early stage of infection by BmDNV-ZJ. The serpin can control the enzymatic activity and regulate the apoptosis, and the thiolperoxiredoxin can prevent oxidation. In the hemolymph, the differentially expressed proteins induced by viral infection may be peroxiredoxin-like protein or mitogen-activated protein kinase. The mitogen-activated protein kinase can regulate apoptosis, and the peroxiredoxin-like protein can eliminate free radicals and prevent oxidation. In these tests, few differentially expressed proteins were acquired at the early stage of infection by BmDNV-ZJ, so, this indicated that there were little actions generated in the body of the silkworm. And the silkworm resist the viral infection through secretion of some protein factors which can anti-oxidize or regulate apoptosis by the target site of infection viral （midgut） and the irnnaunologic tissue （hemolymph）.