Ca^2＋、ABA预处理蝴蝶兰类原球茎的脱水保护系统比较及信号传导关系

Comparison on the Dehydration Protection Systems and Signal Transduction Relationship of CaCl_2 and ABA Pretreatment on the Phalaenopsis PLB

CaCl2和ABA溶液及两者联合分别预处理蝴蝶兰类原球茎(PLB)后脱水,对比PLB脱水前后的成活率、脱水保护系统指标变化及Ca2+与ABA信号传导关系。结果表明,CaCl2和ABA溶液及两者联合预处理均能明显提高PLB脱水后的成活率。CaCl2和ABA溶液对可溶性糖、蔗糖、还原糖、SOD和总抗氧化能力有相同的效应趋势,对可溶性蛋白、热稳定蛋白含量及POD、CAT活性的作用趋势有所差异。ABA溶液提高PLB耐脱水性的能力被胞质Ca2+螯合剂BAPTA/AM和钙调蛋白拮抗剂氯丙嗪所削弱,Ca2+预处理提高PLB耐脱水能力被ABA合成抑制剂环丙嘧啶醇削弱。在蝴蝶兰PLB的耐脱水性诱导中,Ca2+和ABA具有基本相同的生理生化基础,在信号传递中两者互相关联,CaCl2取代ABA用于提高PLB耐脱水性是可行的。

In this paper we pretreated Phalaenopsis PLB （protocorm-like body） with CaCl2, ABA and both, respectively, and compared survival rate, dehydration protection system before and after desiccation, as well as the signal transduction relationship between Ca〉 and ABA. The results showed survival rates of dehydrated PLB pretreated with CaCl2, ABA and both all increased significantly. CaCl2 and ABA exhibited similar impact tendencies on the indexes of soluble carbohydrate, sucrose, reducing carbohydrate, SOD activity and antioxidative capability, respectively, but with respect to the indexes of soluble protein, heat-stable protein, POD and CAT, their impact tendencies showed deviation. The ability that ABA increased the dehydration tolerance of PLB was weaken by promethazine （calmodulin antagonist） or BAPTA/AM （cytosolic calcium chelator）. Ancymidol （inhibitor of ABA synthesis） decreased the effect of ABA on the PLB＇s dehydration tolerance. Due to the fact that the effects of CaCl2 and ABA had almost the same basic of physiology and biochemistry, and that ABA and Ca〉 related to each other by the signal transduction system during dehydration, it is practical to replace ABA with CaCl2 for pretreatment to induce the dehydration tolerance ofPhalaenopsis PLB.