HSPA5基因3′非翻译区多态性与肝细胞癌预后的关系

Association between the polymorphisms in the 3′ UTR of HSPA5 gene and hepatocellular carcinoma prognosis

目的调查70 kDa热休克蛋白5(HSPA5)基因3′非翻译区的变异与肝细胞癌预后的关系。方法从288例肝细胞癌患者外周血中提取DNA,采用TaqMan检测HSPA5基因的rs16927997、rs1140763和rs12009。Kaplan-Meier方法和Log-rank检验用于评价总生存率。结果 HSPA5单体型的分布与临床特性不相关。单变量分析显示等位基因、基因型、单体型和双体型不影响生存。多重比较结果显示临床特征与病人总的预后无关(P>0.05)。结论中国人HSPA5基因3′非翻译区的变异对HCC的预后没有明显影响。

Objective To investigated the possible association between the polymorphisms in the 3′ untranslated region（UTR） of HSPA5 and hepatocellular carcinoma（HCC） prognosis.Methods DNA from blood samples of 288 HCC patients was typed for rs16927997,rs1140763 and rs12009 by TaqMan assays.The Kaplan-Meier method and log-rank test were used to estimate overall survival.Results The distribution of haplotype was not related to the clinical characteristics.Univariate analysis showed that the allele,genetype,haplotype and diplotype did not effect the survival.None of the clinical features show a significant association（P 0.05） with overall patient outcome in multiple comparisons.Conclusion There is no noteworthy influence of 3′ UTR polymorphisms in the HSPA5 on prognosis of HCC in the Chinese population.