恩度对胃癌细胞的增殖抑制效应及机制探讨

Effect and mechanism of Endostar on the proliferation of gastric cancer cell

目的观察人重组血管内皮抑素(恩度)对胃癌细胞NCI-N87增殖、凋亡及侵袭能力的影响,并探讨其机制。方法取贴壁生长的NCI-N87,分为对照组(细胞中不加恩度)和实验组,实验组中分别加入终浓度为50、100、200、400μg/ml的恩度,培养72 h。用MTT比色法检测各组NCI-N87增殖抑制率,流式细胞术和Transwell侵袭试验检测细胞凋亡和侵袭力,Western blot法检测各组细胞中Snail、E-cadherin表达变化。结果恩度可抑制NCI-N87细胞增殖、诱导其凋亡;Transwell小室培养细胞24 h后,对照组NCI-N87穿膜数为(215.65±4.04)个,实验组(121.33±5.86)个(P<0.05);与对照组相比,实验组Snail印迹减弱,E-cadherin印迹增强。结论恩度可抑制人胃癌NCI-N87细胞增殖和侵袭,诱导细胞凋亡,其机制可能与恩度导致细胞中Snail表达下降、E-cadherin表达增强有关。

Objective To observe the effect of recombinant human endostatin （Endostar） on the proliferation, invasion and apoptosis of gastric cancer cell strain NCI-N87, and explore its mechanism. Methods The NCI-N87 cells were divided into control group（ no Endostar） and experiment group（cultured with 50, I00,200,400 txg/ml Endostar respectively）, and were cultured for 72 hours. MTr assay was used to examine the inhibition rate of the NCI-N87 growth; the apoptosis rate was examined by flow cytometry;invasion ability was examined by Transwell invasion assay and MTT assay; Snail and E-cadherin expression in NCI-N87 were examined by Western blot. Results Compared with control group, Endostar could suppress the growth of NCI-N87 and induced it apoptosis. The invasiveness of NCI-N87 was obviously inhibited by Endostar after culture 24 hours. Snail expression was decresed, while E-cadherin expression was increased in the experiment group. Conclusions Endostar can suppress the growth,invasion and induce the apoptosis of NCI-N87 cells in vitro. The machanism may be that Endostar can effect the expression of Snail and E-cadherin of gastric carcinoma cell.