铁皮石斛组培快繁技术

Tissue culture and rapid propagation of Dendrobium officinale

以铁皮石斛无菌苗的茎段为外植体,通过诱导丛生芽,进行壮苗生根,建立了一套快繁体系。结果显示,铁皮石斛试管苗在MS培养基中生长状况最好,当添加NAA的浓度为0.1 mg/L,6-BA的浓度为2.0 mg/L时,铁皮石斛丛生芽的增殖率达到最高。铁皮石斛幼苗壮苗的较佳培养基为MS+0.5 mg/L IBA+0.5 mg/L NAA。同时,单独使用0.5 mg/L NAA的培养基中小苗生根快,根数多。香蕉泥能促进幼苗根系的生长。组培苗移栽到纯树皮的基质中,成活率最高。

An effective method for rapid propagation of Dendrobium officinale was established to induct cluster shoots, strengthening plantlet and rooting. The results showed that： Dendrobium officinale plantlet grew best in MS medium, The proliferation rate of cluster shoots reached the highest in MS medium with NAA 0.1 mg/L and 6-BA 2.0 mg/L. The most suitable medium for strengthening plantlet and taking roots was MS ＋ 0.5 mg/LIBA ＋ 0.5 mg/LNAA. The plantlet rooted rapidly and got more roots with 0.5 mg/L NAA alone in addition. The banana juice could promote growth of plantlet roots. The survival rate was highest when plantlet were transplanted into the matrix of pure bark.