NACT前后宫颈癌组织中PCNA、p53、P-gp的表达变化及意义

Expression and its significance of PCNA,p53,P-gp in cervical carcinoma before and after neoadjuvant chemotherapy

目的探讨宫颈癌新辅助化疗(NACT)前后增殖细胞核抗原(PCNA)、抑癌基因p53和P-糖蛋白(P-gp)在宫颈癌组织中的表达变化及其与化疗疗效的关系。方法对60例局部晚期宫颈癌患者行NACT,采用免疫组化法检测化疗前后宫颈癌组织中的PCNA、p53、P-gp。结果化疗前宫颈鳞癌组织中PCNA、p53和P-gp阳性表达率分别为86.7%、60.0%、56.7%;化疗后分别为93.3%、83.3%、83.3%,化疗前后p53、P-gp阳性率相比,P均<0.05。NACT前宫颈癌组织p53、P-gp表达阴性者NACT有效率与p53、P-gp表达阳性者相比,P均<0.05。结论NACT前后宫颈癌组织中p53、P-gp的表达水平有显著差异,p53、P-gp的表达水平可作为宫颈癌化疗疗效敏感性的预测指标。

Objective To explore the expression of proliferating cell nuclear antigen （ PCNA）, tumor suppressor gene p53 and P-glycoprotein （P-gp） in cervical cancer before and after neoadjuvant chemotherapy, and investigate its relationship with the effect of chemotherapy. Methods 60 patients with locally advanced cervical cancer were treated with neoadjuvant chemotherapy （NACT）. Expression of PCNA, p53, P-gp in cervical cancer before and after chemotherapy were detected by immunohistochemical staining. Results Positive rate of PCNA, p53 and P-gp in cervical cancer before chemotherapy were 86.7%, 60.0%, 56.7%, respectively, after chemotherapy there that 93.3%, 83.3%, 83.3%. Expression of p53, P-pg in cervical cancer before NACT compared with after NACT, all P 〈 0.05. Efficient of NACT in patients with p53, P-pg expression negative before NACT compared to positive, all P 〈 0.05. Conclusions Significantly difference are found in expression levels of p53, P-gp in cervical cancer before and after NACT. Expression levels of p53, P-gp cancer chemotherapy can be used as a predictor of sensitivity.