摘要
主要综述了近年来葡萄胚性愈伤组织的诱导、体细胞胚胎发生的诱导、体细胞胚长期保存和体细胞胚的萌发与成苗的研究进展,并对研究中的主要影响因素进行叙述。此外,还介绍了体细胞胚在转基因上的应用,指出葡萄体细胞胚胎发生过程还存在胚状体诱导难,再生率低等问题。对葡萄胚性悬浮培养液的建立及体细胞胚发生在转基因、原生质体培养等上的应用进行了展望。
The research progress of grapevine embryogenic callus induction, initiation and maintenance of long term somatic embryogenesis, and somatic embryo germination and seedling establishment were reviewed. Their main influence factors were also described. In addition, somatic embryos applied to genetic transformation, the difficult induction of grapevine somatic embryo and low efficiency of plant germination and other issues were introduced. Prospects grapevine embryogenic suspension establishment and somatic embryogenesis used in zenetic transformation and orotoolast culture were also described here.
出处
《中国农学通报》
CSCD
2012年第1期125-132,共8页
Chinese Agricultural Science Bulletin
基金
现代农业产业技术体系专项资金"国家葡萄产业技术体系"(CARS-30)
江苏省科技厅"早熟
抗病
优质葡萄新品种选育"(BE2009314-2)
江苏省农业科技自主创新资金"无核
优质葡萄新品种选育"[cx(10)110]
关键词
葡萄
体细胞胚发生
离体培养
grapevine
somatic embryogenesis
in vitro culture
