摘要
为了提高雪龙黑牛的胴体性状,进行新品种选育,以组织蛋白酶D、L(Cathepsin D,Cathepsin L)基因为候选基因,利用PCR-RFLP方法对114头雪龙黑牛F1代商品牛进行多态性检测,应用SAS9.2软件采用最小二乘拟合线性模型进行分析,结果表明,ctsd、c581t位点与雪龙黑牛的经济性状无明显的相关性,ctsl基因Xma I位点与雪龙黑牛的三角牛腩厚显著相关,且AB型个体三角牛腩厚与AA和BB型个体三角牛腩厚差异显著。
The objective of this study was to explore the genetic polymorphisms of Cathepsin 'D, L and their association with carcass traits. In this experiment, Genetic polymorphisms of the etsd and ctsl Genes were studied by PCR-RFLE and analyzed their association with meat quality traits in Snow ' Dragon black' cattle by SAS 9.2. The results showed that etsd, c581t polymorphism had no associated with economic traits, cts1, xma I polymorphism had significantly associated with sirloin triangle thickness (P〈0.05), sirloin triangle thickness of AB genotype had significant difference with AA and BB genotype.
出处
《中国农学通报》
CSCD
2012年第2期6-9,共4页
Chinese Agricultural Science Bulletin
基金
农业部专项"现代农业(肉牛)产业技术体系岗位科学家"(CARS-38)
转基因生物新品种培育重大专项"优质高效转基因肉牛新品种培育"(2009ZX08007-005B)
"十一五"转基因重大专项"肉牛脂肪沉积相关基因的转基因研究"(2008ZX08007-2)
国家发改委"国家发改委雪龙黑牛分子育种关键技术研究及产业化应用"