羊草叶片SPAD值与叶绿素含量的相关分析

Correlation Analysis Between SPAD Value and Chlorophyll Content of Leaf of Leymus chinensis

为了探讨野外生态学实验采用叶绿素仪简便快速测定叶绿素的精确度，以及72．SPAD值与叶绿素含量的关系。采用SPAD叶绿素仪和酒精．丙酮浸提法对天然草原羊草的叶绿素SPAD值、叶绿素a、叶绿素b和总叶绿素含量进行测定。结果表明，在同一草原不同羊草个体之间羊草叶绿素含量和叶绿素SPAD值在一定范围波动，变异系数为20．457％～36．921％。河北沽源和内蒙古图牧集天然草原羊草叶绿素a、叶绿素b和总叶绿素含量随叶绿素SPAD值的增加而增加，呈线性函数、指数和幂函数规律变化，且均达到极显著相关水平，其中线性函数相关程度最大，可以更准确地预测羊草叶绿素含量。因此，可以利用SPAD．502叶绿素仪在野外条件下原位准确、快速地测定羊草叶绿素，为牧草生产实践提供理论依据。

In order to discuss accuracy of determining chlorophyll with SPAD chlorophyll meter in the eecological field experiments, SPAD value, chlorophyll a, chlorophyll b and total chlorophyll content of Leymus cbinensis on natural grassland were determined with SPAD chlorophyll meter and alcohol-acetone extraction method. The results showed that chlorophyll content and SPAD value of L. cbinensis on different grasslands were changed in the certain range. Coefficient of variation was range from 20.457% to 36.921%. Regression analysis showed that relationships between chlorophyll a content, chlorophyll b content, total chlorophyll content and SPAD value of L. cbinensis on Inner Mongolia Tumuji and Hebei Guyuan natural grassland were significant linear, index and power function. And the coefficient of determination of linear function was highest than those of index and power function. Linear function could greatly reflect the relationships between chlorophyll content and SPAD value of L. chinensis. Chlorophyll content of L. chinensis can be accurately determined on field condition with SPAD chlorophyll meter. This method is simple and fast on field experiment, providing theoretical basis for grass production.