过瘤胃胆碱对泌乳奶牛血液脂质代谢和抗氧化应激的影响

Blood Lipid Profiles and Anti-oxidative Stress for Lactating Cows Fed Rumen-protected Choline

试验旨在研究过瘤胃胆碱(RPC)对泌乳奶牛血液脂质代谢和抗氧化应激的影响。选用10头泌乳盛期奶牛为试验动物,采用自身对照试验设计,试验时间分为对照期、试验1期、试验2期共36天,每期12天。RPC的添加水平为每头40g/天。每期采集血液制备血浆,测定其脂质代谢和抗氧化应激指标。结果表明,与对照期相比,日粮添加RPC对血浆肌酐、甘油三脂影响不大,差异不显著(P>0.05);可降低血浆尿素氮水平(P<0.05),增加血浆总胆固醇水平(P<0.05)。血浆总抗氧化能力、超氧化物歧化酶、丙二醛、谷胱甘肽-过氧化物酶水平均不受RPC的影响(P>0.05)。综上所述,RPC有利于改善泌乳盛期奶牛脂质代谢和蛋白质利用效率,而对抗氧化应激影响不大。

This study was conducted to evaluate the blood lipid profiles and anti-oxidative stress for lactating cows fed rumen-protected choline （RPC）. Ten cows in early lactation were used as the experimental animal with self-pair design. Trial lasted for 3 durations of 12 days including control, trial 1, and trial 2, respectively. Each cow was supplemented with a daily dose of 40 g RPC. Blood was collected to harvest plasma for measurements of blood lipid profiles and anti-oxidant stress. Feeding of RPC did not affect plasma, creatinine, and triglyceride levels （P〉0.05）. However, plasma urea nitrogen was lower for trial 1 and 2 phases than control （P〈0.05）; and plasma cholesterol in trial 1 and 2 duration was higher relative to control （P〈0.05）. Compared with control, plasma concentrations of total anti-oxidative capacity, superoxide dismutase, malondialdehyde, and glutathione peroxidase was not altered for RPC supplementation （P〉0.05）. These results indicated that, under the present experimental condition, dietary inclusion of RPC was beneficial for protein utilization and blood lipid metabolism, but not for anti-oxidative stress for peak-lactating dairy cows.